WISCONSIN LONGITUDINAL STUDY

LIFE HISTORIES AND HEALTH IN MIDLIFE STUDY (1996-1998)

PROTOCOL FOLLOWED AT GENERAL CLINICAL RESEARCH CENTER

1. Admit to GCRC at approximately noon for 1-night admission.

2. Check vital signs: oral temperature, pulse, blood pressure.

Obtain 3 seated blood pressure and pulse after 5 minutes of quiet sitting (using approximate cuff size on eft and right arms). Also record the average of the two Blood Pressures that are most similar.

3. Record height and weight. Measure waist and hip as follows:

Waist Circumference - Have the participant stand erect. A cloth tape measure will be used. Make measurements when they are wearing only one layer of clothing. Place tape around narrowest point between ribs and iliac crest. Be sure the tape measure goes around evenly (parallel to the floor). Record measurement to the nearest tenth of an inch.

1^st Hip Circumference - Have participant stand erect. Ask patient to point to their hip bone. Place the tape measure at the iliac crest and wrap around body. Make sure the measure is parallel to the floor. Record measurement to the nearest tenth of an inch.

2^nd Hip Circumference - have participant stand erect. Place the tape measure at the maximum buttock diameter and wrap it around the body. Stand to the side of the participant to see that tape is placed at point of maximum buttocks extension. Record measurement to the nearest tenth of an inch.

4. Obtain blood samples prior to 0700 on Day 2. Requested laboratory tests: HDL Cholesterol Calcium Total Protein

DHEA-S Magnesium Albumin

Hemoglobin A1C Phosphate Bili, Total

Electrolytes and CHEM7 Uric Acid GGT

CBC Chol, Tot Alkphos

Westergren sedimentation LD, Total AST

5. Obtain three saliva samples of 2-3 cc/each. Samples to be collected once before dinner on Day 1, immediately before bedtime on Day 1, and first thing in the a.m., Day 2. Test tubes will be supplied by researchers, and will be collected by researchers on Day 2. GCRC must freeze samples immediately after taking them.

6. General diet. No ETOH. NPO except for water after midnight, the night before the blood draw. Vouchers will be provided for lunch and dinner of Day 1.

7. Obtain a 12-hour urine specimen, and complete log for urine collection. No need for HCL or Boric Acid as long as the urine is refrigerated. The urine specimen must have written on its container cap "PROCESS IMMEDIATELY." The specimen should be delivered to Specimen Control (B4/236) at end of collection. Laboratory tests requested are:

Cortisol Creatinine

Fractionated Catecholamine (epinephrine/norepinephrine/dopamine)

Send to hospital lab.

8. Provide patient with breakfast if desired.

9. Patient may take own medications.10. Acetaminophen, 650 mg. po q4h prn pain.

11. In a.m. of Day 2, patient will receive physical health examination by Dr. Muller.

12. Discharge patient in a.m. after physical health exam or after completion of 12-hour urine specimen.

**** Patient will leave GCRC in afternoon of Day 1 to participate in laboratory study of emotions in Department of Psychology. Transportation will be arranged by the investigators. Patient may eat dinner in the cafeteria or have a pass to eat outside the hospital.

All questionnaire and sleep cap instruction will be provided by study personnel.